The costitution changes of glucose mother liqour under different glucose mother liquor concentration with glucoamylase as catalyst were researched. The results show that: with the increase of reaction solution concentration, the trend of glucose to maltose and isomaltose becomes strong. And the ratio of isomaltose to maltose is changed. The initial ratio of isomaltose to maltose is 0.49,and the concentration of isomaltose is 7.16%. Isomaltose concentration becomes 7.16% after reaction under 60 ℃ waterbath for 72h, and isomaltose concentration is 17.33%. The isomaltase concentration increases by 242% after condensation of glucose mother liquor.