Objective: To investigate the serum procalcitonin(PCT) and C-reactive protein(CRP) in the diagnosis of infectious diseases.Method: A retrospective analysis was designed to explore the relationship of procalcitonin and C-reactive protein levels and Infectious disease.All the cases were confirmed by clinical trials of pathogenic examination in our hospital clinical laboratory.Result: The level of procalcitonin and C-reactive protein in serum is related to the severity of the infection disease.The concentration of procalcitonin between bacterial infection group and normal control group was different statistically significant(P 0.05);the concentration of procalcitonin in the bacterial infection group compared to the original with the viral infection group,the difference was statistically significant(P 0.05);the concentration of procalcitonin in the infection group and normal control group was no significant difference(P 0.05).C-reactive protein compared among the three groups,were statistically significant(P 0.05).Conclusion: Using the serum procalcitonin and C-reactive protein in the detection of clinical infection diagnosis can improve the accuracy of the diagnosis in the infectious diseases.