Supercritical fluid chromatography is not (only) normal-phase chromatography

Supercritical fluid chromatography (SFC), now using carbon dioxide as a major component of the mobile phase, has been known for over 60 years but still some misunderstandings remain about its capabilities. Among them, SFC is often described as a normal-phase chromatographic technique, based on different considerations: polarity of the stationary phase, elution order of the analytes, relative non-polarity of the mobile phase, non-linear retention behaviour, or adsorption retention mechanisms. All of these assumptions are true to a certain extent, and in certain circumstances. But also, all of these assumptions are wrong in different circumstances. In this paper, the criteria to categorize SFC as a normal-phase chromatographic method will be examined individually, considering all knowledge acquired from the early years of its development. Finally, it will appear that the “normal-phase” glass lens is greatly reducing the true extent of SFC's possibilities.