Wheat protoplast preparation and transformation in a 96-well plate v1

This protocol details a robust, 96-well plate–based workflow for the isolation and PEG–Ca²⁺ transfection of wheat leaf protoplasts, enabling high-throughput screening. The miniaturised format standardises cell normalisation, PEG exposure, and plate handling, and is compatible with luciferase reporters for ~16 h readouts