化学
细胞外小泡
适体
乳腺癌
癌症
检出限
CD63
离心
计算生物学
色谱法
纳米技术
分子生物学
微泡
生物化学
细胞生物学
内科学
基因
小RNA
医学
材料科学
生物
作者
Xiaohui Chen,Yun Deng,Ruyan Niu,Zixin Sun,Alya Batool,Liu Wang,Chong Zhou,Ningyu Ma,Qiya Yang,Guoxiang Liu,Jichun Yang,Yang Luo
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-08-18
卷期号:94 (38): 13019-13027
被引量:10
标识
DOI:10.1021/acs.analchem.2c01683
摘要
Cancer-derived small extracellular vesicles (csEVs) play critical roles in the genesis and development of various cancers. However, accurate detection of low-abundance csEVs remains particularly challenging due to the complex clinical sample composition. In the present study, we constructed a Programmable Isothermal Cascade Keen Enzyme-free Reporter (PICKER) for the reliable detection and acquisition of the relative abundance of csEVs in total sEVs (tsEVs) by integrating dual-aptamer recognition (cancer-specific protein EpCAM and tetraspanin protein CD63) with a catalytic hairpin assembly (CHA) amplification. By employing this strategy, we were able to achieve a detection limit of 420 particles/μL csEVs. Particularly, we proposed a novel particle ratio index of csEV against tsEV (PRcsEV/tsEV) to greatly eliminate errors from inconsistent centrifugation, which was calculated from the fluorescence ratio produced by csEVs and tsEVs. The PICKER showed a 1/10,000 discrimination capability by successfully picking out 1.0 × 103 csEV from 1.0 × 107 tsEV per microliter. We also found that the PRcsEV/tsEV value increased proportional to the stages of breast cancer by analyzing EVs from clinical patients’ plasma. Taken together, we established a PICKER strategy capable of accurately discriminating csEVs, and the proposed PRcsEV/tsEV had been proven a potential indicator of breast cancer staging, paving the way toward facilitating cancer diagnosis and precision therapeutics.
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