A new set of polymorphic simple sequence repeat (SSR) markers from a wild strawberry (Fragaria vesca) are transferable to other diploid Fragaria species and to Fragaria × ananassa

Abstract A set of 41 polymorphic microsatellite markers were developed using a CT/AG‐enriched genomic library of Fragaria vesca cv. Reine des Vallées. Thirty‐five of them were polymorphic in F. vesca and were tested in one accession each of six additional diploid Fragaria species and the octoploid Fragaria × ananassa . A mean of 5.3 alleles per locus and a low level of observed heterozygosity were generally detected in the 32 single‐locus simple sequence repeats of F. vesca . Most of these loci amplify in the other diploid species and in F. × ananassa.