乙型肝炎病毒
肝细胞癌
生物标志物
结合珠蛋白
生物
免疫学
蛋白质组学
肝病
发病机制
乙型肝炎
病毒学
血清学
转甲状腺素
载脂蛋白A1
病毒
蛋白质组
载脂蛋白B
抗体
生物信息学
癌症研究
基因
生物化学
胆固醇
内分泌学
作者
Qing‐Yu He,George Lau,Yuan Zhou,Siu‐Tsan Yuen,Marie C.M. Lin,Hsiang‐Fu Kung,Jen‐Fu Chiu
出处
期刊:Proteomics
[Wiley]
日期:2003-05-01
卷期号:3 (5): 666-674
被引量:147
标识
DOI:10.1002/pmic.200300394
摘要
Hepatitis B virus (HBV), a serious infectious and widespread human pathogen, represents a major health problem worldwide. Chronic HBV infection has a very high risk of evolving into hepatocellular carcinoma. Although considerable progress was made during the recent past, the pathogenesis of HBV infection is still elusive and a definite diagnosis of HBV infected liver information still relies on biopsy histological test. In this report, we used proteomics technology to globally examine HBV infected serum samples aiming at searching for disease-associated proteins that can be used as serological biomarkers for diagnosis and/or target proteins for pathogenetic study. By comparing with normal and HBV negative serum samples, we found that at least seven proteins were significantly changed in HBV infected sera. These greatly altered proteins were identified to be haptoglobin beta and alpha2 chain, apolipoprotein A-I and A-IV, alpha1-antitrypsin, transthyretin and DNA topoisomerase IIbeta. The alteration of these proteins is displayed not only in quantity but also in patterns (or specificity), which can be correlated with necroinflammatory scores. In particular, apolipoprotein A-I presents heterogeneous change in expression level with different isoforms and alpha1-antitrypsin produces evidently different fragments implying diverse cleavage pathways. These unique phenomena appear specific to HBV infection. A combination simultaneously considering the quantities and isoforms of these proteins could be a useful serum biomarker (or index) for HBV diagnosis and therapy.
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