Rare earth ions enhanced near infrared fluorescence of Ag2S quantum dots for the detection of fluoride ions in living cells

In this work, a novel phenomenon was discovered that the fluorescence intensity of silver sulfide quantum dots (Ag₂S QDs) could be enhanced in the presence of rare earth ions through aggregation-induced emission (AIE). Based on the strong coordination between rare earth ions and F^-, a facile and label-free strategy was developed for the detection of F^- in living cells. Ag₂S QDs were synthesized using 3-mercaptopropionic acid as sulfur source and stabilizer in aqueous solution. The near infrared (NIR) emitting QDs exhibited excellent photostalilty, high quantum yield and low toxic. Interestingly, the fluorescence intensity of QDs was obviously enhanced upon the addition of various rare earth ions, especially in the presence of Gd³⁺. The AIE mechanism was proved via the TEM, zeta potential and dynamic light scattering analysis. Moreover, the coordination between rare earth ions and F^- could lead to the quenching of fluorescence QDs due to the weakening the AIE. Based on these findings, we developed a highly sensitive and selective method for detection of F^-. The label-free NIR fluorescence probe was successfully used for F^- bioimaging in live cells.