病毒学
水泡性口炎病毒
中和
病毒
单克隆抗体
弹状病毒科
抗体
中和抗体
生物
单反病毒
效力
冠状病毒
2019年冠状病毒病(COVID-19)
副粘病毒科
医学
病毒性疾病
免疫学
体外
传染病(医学专业)
疾病
病理
生物化学
作者
Jianhui Nie,Qianqian Li,Jiajing Wu,Chenyan Zhao,Huan Hao,Huan Liu,Li Zhang,Lingling Nie,Haiyang Qin,Meng Wang,Qiong Lu,Xiaoyü Li,Qiyu Sun,Junkai Liu,Chen Fan,Weijin Huang,Miao Xu,Youchun Wang
出处
期刊:Nature Protocols
[Springer Nature]
日期:2020-09-25
卷期号:15 (11): 3699-3715
被引量:296
标识
DOI:10.1038/s41596-020-0394-5
摘要
Pseudotyped viruses are useful virological tools because of their safety and versatility. On the basis of a vesicular stomatitis virus (VSV) pseudotyped virus production system, we developed a pseudotyped virus-based neutralization assay against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in biosafety level 2 facilities. Compared with the binding antibody test, the neutralization assay could discriminate the protective agents from the antibody family. This protocol includes production and titration of the SARS-CoV-2 S pseudotyped virus and the neutralization assay based on it. Various types of samples targeting virus attachment and entry could be evaluated for their potency, including serum samples derived from animals and humans, monoclonal antibodies and fusion inhibitors (peptides or small molecules). If the pseudotyped virus stock has been prepared in advance, it will take 2 days to get the potency data for the candidate samples. Experience in handling cells is needed before implementing this protocol.
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