生物传感器
材料科学
纳米技术
量子点
光电流
猝灭(荧光)
纳米颗粒
纳米化学
共价有机骨架
原位
适体
信号(编程语言)
共价键
荧光
光电子学
多孔性
化学
计算机科学
光学
有机化学
复合材料
程序设计语言
物理
生物
遗传学
作者
Xiaoshi Yan,Hongkun Li,Tengyue Yin,Guifen Jie,Hong Zhou
标识
DOI:10.1016/j.bios.2022.114694
摘要
In this work, a new photoelectrochemical (PEC) biosensing platform based on an ordered two-dimensional (2D) ultrathin covalent organic framework (COF) film and AgInS2 quantum dots (QDs) has been developed to enable dual-target detection of HIV and CEA. The porous COF film was firstly in situ generated on ITO, displaying super-stable and intense photocurrent with excellent repeatability. Moreover, an effective PEC quenching probe was specifically designed by loading large number of AgInS2 QDs on Au nanoparticles (NPs). After target HIV-induced cyclic amplification process to generate abundant DNA S0, the Au NPs-AgInS2 QDs probe was binded to the COF film through DNA hybridization, enabling PEC signal of the COF film to turn "off" for ultra-sensitive detection of HIV. Furthermore, when CEA as the second target specifically binded to its aptamer, the Au NPs-AgInS2 QDs quenching probe was released, achieving PEC signal "on" of the T-DA COF film for ultra-sensitive detection of CEA. This work opened a unique 2-D COF film-based PEC biosensing platform with excellent signal for rapid detection of dual targets, which can effectively avoid false positives and negatives and shows promising application for early prevention and detection of cancer diseases.
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