基因组
基因组编辑
计算生物学
生物
计算机科学
病毒学
遗传学
基因
作者
Yong Shen,Ye Tao,Zihan Li,Torotwa Herman Kimutai,Hao Song,Xiaoou Dong,Jianmin Wan
出处
期刊:aBIOTECH
[Springer Nature]
日期:2024-05-08
标识
DOI:10.1007/s42994-024-00147-7
摘要
Abstract Genome editing holds great promise for the molecular breeding of plants, yet its application is hindered by the shortage of simple and effective means of delivering genome editing reagents into plants. Conventional plant transformation-based methods for delivery of genome editing reagents into plants often involve prolonged tissue culture, a labor-intensive and technically challenging process for many elite crop cultivars. In this review, we describe various virus-based methods that have been employed to deliver genome editing reagents, including components of the CRISPR/Cas machinery and donor DNA for precision editing in plants. We update the progress in these methods with recent successful examples of genome editing achieved through virus-based delivery in different plant species, highlight the advantages and limitations of these delivery approaches, and discuss the remaining challenges.
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