电穿孔
清脆的
生物
基因组编辑
Cas9
基因
基因表达
功能(生物学)
信使核糖核酸
计算生物学
遗传增强
体外
分子生物学
遗传学
作者
Federica Nardi,Laura Pezzella,Roberta Drago,Anthea Di Rita,Martina Simoncelli,Giuseppe Marotta,Alessandro Gozzetti,Monica Bocchia,Anna Kabanova
标识
DOI:10.1002/eji.202149784
摘要
Robust methods for manipulation of human B cells, isolated from healthy donors and patients with B cell disorders, has the potential to significantly accelerate B cell research. Our work describes a step-by-step protocol to perform electroporation-based screening of gene function in B cells through the use of Cas9 ribonuclecomplexes and in vitro produced mRNA.
科研通智能强力驱动
Strongly Powered by AbleSci AI