[Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua].

OBJECTIVE: Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua. METHOD: A P450 cDNA gene was cloned from A. annua by RT-PCR. The bioinformatics analysis of the P450 gene was performed. RESULT: The complete ORF of this P450 cDNA is 1 464 bp and encodes 488 aa. The sequence was reported to GenBank and coded as DQ667171. Bioinformatics analysis of the P450 cDNA showed it encoded an A-type P450 protein with 54. 992 kDa, it's isoelectric point was 8.83 and the possibility of export to mitochondria was 0.893 2. CONCLUSION: The comparable analysis of the P450 with CYP71AV1 revealed that the two proteins probably performed the same function because of the similar character.