病毒
黄瓜花叶病毒
病毒学
生物
实时聚合酶链反应
斑驳
核糖核酸
生物测定
聚合酶链反应
植物病毒
基因
生物化学
遗传学
作者
Sang-Min Kim,Sang-Hyun Nam,Jung Myung Lee,Kyu-Ock Yim,Kook‐Hyung Kim
出处
期刊:Molecules and Cells
[Springer Science+Business Media]
日期:2003-12-01
卷期号:16 (3): 338-342
被引量:10
标识
DOI:10.1016/s1016-8478(23)13810-6
摘要
Heat treatment is commonly used to control viral contamination of seeds. To study virus inactivation, virus was purified from seeds contaminated with Cucumber green mottle mosaic virus (CGMMV) after various heat treatments. CGMMV particles were observed to be physically disrupted by high temperature. Analysis of viral RNA revealed that the 5′and 3′ termini of the genome were protected whereas regions between 2–2.5, 3.2–3.7 and 4–4.8 kb from the 5′terminus were not. Heat inactivation of virus on seeds was confirmed by RT-PCR using primers for a heat-sensitive region. The RT-PCR approach developed here may prove preferable to time- and labor-intensive bioassays for assessing virus heat inactivation.
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