基因组
DNA测序
计算生物学
2019年冠状病毒病(COVID-19)
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
生物
鉴定(生物学)
冠状病毒
2019-20冠状病毒爆发
基因
病毒学
遗传学
医学
传染病(医学专业)
内科学
植物
爆发
疾病
出处
期刊:Chinese Journal of Laboratory Medicine
日期:2020-03-11
卷期号:43 (03): 217-220
标识
DOI:10.3760/cma.j.issn.1009-9158.2020.03.004
摘要
As one of the two methods for 2019 novel coronavirus (2019-nCoV), gene sequencing is different from quantitative real-time PCR (RT-PCR) in detection principles. Therefore, gene sequencing has its own pros and cons in clinical application. Currently, metagenomic next-generation sequencing (mNGS) is the most commonly used technology in clinical application.Due to its broad coverage of all types of pathogens, mNGS demonstrates incomparable advantage in rapid identification of novel pathogens such as 2019-nCoV. In addition, it can simultaneously identify other pathogens except 2019-nCoV and mixed infections. On the other hand, however, due to the complexity of mNGS and long detection time, it is unlikely to achieve the purpose of wide-range and rapid diagnosis of 2019 n-CoV. Therefore, mNGS can complement RT-PCR to achieve best clinical application.
Key words:
Coronavirus; Severe acute respiratory syndrome; Nucleic acids; High-throughput nucleottide sequencing; Metagenomics
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