三种生防细菌对新疆灰霉病菌(Botrytis cinerea)的拮抗作用

【Objective】 A study on the biocontrol efficacy of the Bacillus strains A57, A178 and N1729 against 13 Botrytis cinerea isolates from the different area in Xinjiang, was performed for evaluating the future application significance of the three strains for the biological control of plant diseases, and providing scientific basis for the future research on their development. 【Method】 The antagonism of three biocontrol bacteria were tested on PDA plate against 13 Botrytis cinerea isolates respectively. By the liquid fermenting and the tiny hole filters, the antagonism of free cell filtrates were detected on PDA plate. 【Result】 The results demonstrated, the antagonistic ability of A178 strain was the strongest among three biocontrol bacteria, the average inhibition rate of the strain was 45.67%. The average inhibition rate of N1729 strain' free cell filtrates was 44. 61%. A57 strain had the better antagonistic ability to the pathogen, but its free cell filtrates had no effect on the pathogen. 【Conclusion】 These evidences showed that the two strains A178, N1729 were excellent biocontrol material against Botrytis cinerea with good prospects of development and application.