Comparison Effect of Clotrimazole and Silver Nanoparticles on Epithelial of Vagina in the Treatment of Vaginal Candidiasis in mice

Vaginal candidiasis is one of infections occurred in the vagina and cause by Candida that is kind of yeasts.This disease transferable between male and female and cause disease in genital tract in both of them.Studies reported secondary infertility by this infection in genital tract and to be associated with low birth weight and premature birth.Major diseases in candidaisis caused by candida albicanse species that is opportunistic yeast in form of simple superficial infection to acute systemic infection and has been the fourth leading cause of death with infection disease.This disease is becoming resistant to commonly used drugs.With the development of nano technology, nanoparticles have become one of the promising and useful antibacterial agents.Silver nanoparticles (SNPs) have anti fungal, anti viral and anti bacterial properties.Therefore, in this study after microbiological analyses on Candida albicans species that obtain from the Iranian Research Organization for Science and Technology and patient, antifungal susceptibility of clotrimazole and SNPs on two Candida albicans strains-including standard and pathogenic-were evaluated by the broth microdilution method for determining MIC(minimum inhibitory concentration) and using sabaro dextros agar for determining MFC(minimum fungicidal concentration).MIC and MFC of SNPs and clotrimazole were administered to mice that have vaginal C. albicans infection.Our results revealed treatment with clotrimazole decrease the Cellular connections in cornify layer in vaginal epithelial that weren't observed in treatment with SNPs and negative control groups.Therefore according to our finding.Silver nanoparticles have antifungal activity and is also very effective against vaginal candidiasis caused by candida albicans and didn't have harmful effects on vaginal epithelium in comparison with clotrimazole, can be used in the treatment of this disease.