The optimal conditions of arbutin and hydroquinone extraction from plant raze, materials and biologically active supplements based on Vaccinium vitis-idaea and Arctostaphylos uva-ursi leaves were developed. 25% ethanol was used for extraction in quantitation. Based on isocratic fluorometric HPLC chromatographic method of arbutin and hydroquinone detection in Vaccinium vitis-idaea and Arctostaphylos uvaursi leaves was developed. Fluorometric detector increases selectivity and sensitivity of the method. Detection limit averaged 0,1 mg/kg.