Two species of basidiomycetes, Lentinula boryana and Lentinula edodes, were evaluated for their antibacterial activities, biomass production and growth in two different culture media. The basidiomycete L. boryana occurs naturally in the Brazilian territory whilst L. edodes grows in Asia. Mycelia from each species were incubated in liquid media for 28 days and vacuum-filtered. L. boryana showed the largest biomass production in both culture media when compared to L. edodes, which presented significant differences in growth when cultivated in different culture media. Antibacterial activity of the two species was evaluated against 10 bacterial species, six of them being of clinical importance. Both basidiomycetes L. boryana and L. edodes showed antibacterial activity against B. cereus and S. aureus, although only L. edodes was active