Extracellular Shed Vesicle Isolation - InnovaPrep® Concentrating Pipette, Ultracentrifugation, and Systems Biosciences ExoQuick®

Isolation and purification techniques for functional exosomes are limited, and we see a pressing need for methods optimized for basic science applications. Widely used exosome purification techniques using immunological markers are ill-suited for fundamental exosome research since they inherently select for specific subsets of exosomes, limiting the ability to draw general conclusions and interrogate the full spectrum of exosomes produced by various cells. Additionally, removing attached antibodies is challenging, thus there is need for label free vesicle purification for functional vesicle recovery. Furthermore, high forces generated using ultracentrifugation, the gold standard for exosome concentration, can affect exosome morphology, and is expected to negatively impact exosome function.