骨肉瘤
癌症研究
MCL1
小RNA
竞争性内源性RNA
下调和上调
体内
细胞生长
生物
体外
荧光素酶
功能(生物学)
细胞生物学
医学
长非编码RNA
细胞培养
转染
基因
生物化学
遗传学
作者
Xinyu Tan,Dandan Tan,Haomiao Li,Ye Lin,Zhishen Wen,Canjun Zeng
出处
期刊:Current Cancer Drug Targets
[Bentham Science]
日期:2020-04-30
卷期号:20 (4): 288-294
被引量:11
标识
DOI:10.2174/1568009619666191107140948
摘要
Recent studies have reported the vital roles of circular RNAs (circRNAs) in tumor progression. However, the function and expression profile of most circRNAs in osteosarcoma remain unclear.We examined the expression of circEPSTI1, a circRNA, in 50 paired adjacent normal tissues and osteosarcoma tissues by qRT-PCR. Then, we further explored the function of circEPSTI1 in osteosarcoma progression in vitro and in vivo. For example, cell proliferation and migration were examined. Some experiments were performed to explore the regulatory function of circEPSTI1 in miRNA and to investigate the potential role of circEPSTI1 in osteosarcoma.We found that circEPSTI1 was significantly upregulated in osteosarcoma. Inhibition of circEPSTI1 suppressed the osteosarcoma cancer cell proliferation and migration in vitro. Dual luciferase reporter assay showed that circEPSTI1 and MCL1 (myeloid cell leukaemia 1) could bind to miR-892b and that MCL1 and circEPSTI1 were targets of miR-892b.Thus, the circEPSTI1-miR-892b-MCL1 axis affected osteosarcoma progression through the miRNA sponging mechanism. circEPSTI1 may serve as a target and biomarker for osteosarcoma treatment.
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