适体
化学
微流控
聚二甲基硅氧烷
拉曼散射
分析物
纳米技术
检出限
生物传感器
微流控芯片
拉曼光谱
色谱法
材料科学
光学
物理
生物
生物化学
有机化学
遗传学
作者
Cuicui Fu,Yi Wang,Gang Chen,Lina Yang,Shuping Xu,Weiqing Xu
标识
DOI:10.1021/acs.analchem.5b02508
摘要
A surface-enhanced Raman scattering (SERS) measurement of 3,3',4,4'-tetrachlorobiphenyl (PCB77) with aptamer capturing in a microfluidic device was demonstrated. To construct the microfluidic chip, an ordered Ag nanocrown array was fabricated over a patterned polydimethylsiloxane (PDMS) that was achieved by replicating an anodic aluminum oxide (AAO) template. The patterned PDMS sheet was covered with another PDMS sheet having two input channel grooves to form a close chip. The Ag nanocrown array was used for the SERS enhancement area and the detection zone. PCB 77 aptamers were injected into one channel and the other allows for analytes (PCBs). The mercapto aptamers captured the targets in the mixed zone and were immobilized to the SERS detection zone via S-Ag bonds so as to further improve both the SERS sensitivity and selectivity of PCB77. Such an aptamer-based microfluidic chip realized a rapid SERS detection. The lowest detectable concentration of 1.0 × 10(-8) M was achieved for PCB77. This work demonstrates that the aptamer-modified SERS microfluidic sensor can be utilized for selective detections of organic pollutants in the environment.
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