Improved Cell Adhesion on Self‐Assembled Chiral Nematic Cellulose Nanocrystal Films

Abstract Chirality is ubiquitous in nature, and closely related to biological phenomena. Nature‐originated nanomaterials such as cellulose nanocrystals (CNCs) are able to self‐assemble into hierarchical chiral nematic CNC films and impart handedness to nano and micro scale. However, the effects of the chiral nematic surfaces on cell adhesion are still unknown. Herein, this work presents evidence that the left‐handed self‐assembled chiral nematic CNC films (L‐CNC) significantly improve the adhesion of L929 fibroblasts compared to randomly arranged isotropic CNC films (I‐CNC). The fluidic force microscopy‐based single‐cell force spectroscopy is introduced to assess the cell adhesion forces on the substrates of L‐CNC and I‐CNC, respectively. With this method, a maximum adhesion force of 133.2 nN is quantified for mature L929 fibroblasts after culturing for 24 h on L‐CNC, whereas the L929 fibroblasts exert a maximum adhesion force of 78.4 nN on I‐CNC under the same condition. Moreover, the instant SCFS reveals that the integrin pathways are involved in sensing the chirality of substrate surfaces. Overall, this work offers a starting point for the regulation of cell adhesion via the self‐assembled nano and micro architecture of chiral nematic CNC films, with potential practical applications in tissue engineering and regenerative medicine.