细胞外小泡
伤口愈合
巨噬细胞
细胞生物学
巨噬细胞极化
化学
细胞外
小泡
表型
再生(生物学)
细胞
间充质干细胞
膜联蛋白A1
胞外囊泡
M2巨噬细胞
膜联蛋白
体外
微泡
生物化学
生物
免疫学
膜
小RNA
基因
作者
Hao Deng,Yuheng Jiang,Junhao Deng,Fei‐Fan Chang,Junyu Chen,Xiaofeng Sun,Dongliang Cheng,Zhongqi Wang,Ran Li,Jiang Liu,Yang Li,Licheng Zhang,Pengbin Yin
出处
期刊:Biomaterials Science
[The Royal Society of Chemistry]
日期:2024-01-01
卷期号:12 (7): 1761-1770
摘要
The conundrum of wound healing has transformed into an imminent medical challenge. Presently, cell-free therapy centered around extracellular vesicles (EVs) has become a pivotal and promising research avenue. EVs generated from three-dimensional (3D) cell cultures have been previously established to possess enhanced tissue regeneration potential, although the underlying mechanisms remain elusive. In this study, we observed higher expression of annexin ANXA1 in 3D-cultured EVs. Remarkably, 3D-EVs with elevated ANXA1 expression demonstrated a more potent capacity to promote macrophage polarization from the M1 phenotype to the M2 phenotype. Concurrently, they exhibited superior abilities to enhance cell migration and tube formation, facilitating expedited wound healing in animal experiments. Conversely, the application of an ANXA1 inhibitor counteracted the positive effects of 3D-EVs. Taken together, our data validate that extracellular vesicles derived from 3D-cultured MSCs regulate macrophage polarization via ANXA1, thereby fostering wound healing.
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