蛹虫草
虫草素
氧化应激
活性氧
抗氧化剂
细胞外基质
自噬
伤口愈合
弹性蛋白
化学
细胞生长
冬虫夏草
细胞生物学
生物化学
生物
细胞凋亡
免疫学
食品科学
遗传学
作者
Nawapol Upatcha,Palakorn Kaokaen,Natchadaporn Sorraksa,Ruchee Phonchai,Phongsakorn Kunhorm,Nipha Chaicharoenaudomrung,Parinya Noisa
标识
DOI:10.1080/02652048.2023.2198008
摘要
Oxidative stress from reactive oxygen species is the main cause of skin ageing. Cordycepin, a bioactive compound of Cordyceps militaris, contains antioxidant activity. This study examined extracellular matrix, antioxidant effect, autophagy activity, and skin regeneration in human dermal fibroblasts (HDFs) under normal and oxidative stress conditions. Slow disintegration was used to create nano-encapsulated cordyceps extract. HDFs were cultured and treated with 1 M cordycepin, 1 M medium, 0.1 M cordyceps medium loaded nanoparticles (CMP), or 1 mM H2O2. HDFs’ senescent phenotypes were assessed, including cell proliferation, ROS scavenging, collagen and elastin synthesis, antioxidant activity, and wound healing. CMP size averaged 184.5 ± 95.2 nm increased cell proliferation and reduced H2O2-induced ROS. Thus, HDFs treated for 48 h increased skin regeneration activity 2.76-fold by expressing extracellular matrix and rescuing H2O2-induced damaged cells. It was significant that this CMP inhibited H2O2-induced oxidative stress and induced autophagy to regenerate HDFs. The developed CMP could be used in cosmetics.
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