A novel constructed carbonate-mineralized functional bacterial consortium for high-efficiency cadmium biomineralization

A stable, urease-producing consortium (UPC) was constructed for high-efficiency cadmium (Cd) ion mineralization via a short-term and efficient acclimation process (five acclimation transfers). 16S rRNA gene high-throughput sequencing and quantitative polymerase chain reaction (qPCR) analyses of the urease subunit C (ureC) gene suggested that the three functional genera, all belonging to the phylum Firmicutes, rapidly increased during the process and finally composed the UPC (70.22-75.41 % of Sporosarcina, 13.83-20.66 % of norank_f_Bacillaceae, and 5.91-13.69 % of unclassified_f_Bacillaceae). The UPC exhibited good adaptability to a wide range of environmental conditions (a pH range of 4.0-11.0, temperature range of 10-45 °C, and Cd concentration range of 0-200 mg L-1). After 8 h of incubation, 92.87 % of Cd at an initial concentration of 100 mg L-1 was mineralized by UPC, exhibiting a great improvement as compared to the first acclimated consortium (C-1). Furthermore, although the acclimated consortium had been successively transferred 21 times, the Cd biomineralization efficiency remained stable, and this was consistent with the observed stable microbial community structure. X-ray diffraction (XRD) spectra revealed that Cd was mineralized in a (Ca0.67, Cd0.33)CO3 phase. This research obtained a promising microbial resource for the biomineralization of Cd or other hazardous heavy metal contaminants.