核苷
嘧啶
化学
寡核苷酸
DNA
复式(建筑)
立体化学
组合化学
序列(生物学)
核苷类似物
生物化学
作者
Ryotaro Notomi,Lei Wang,Takayuki Osuki,Hirokazu Okamura,Shigeki Sasaki,Yoshihiro Taniguchi
标识
DOI:10.1016/j.bmc.2020.115782
摘要
The triplex DNA forming method is an attractive tool as a gene-targeting agent. Using artificial nucleoside analogues based on C-nucleoside, stable and selective triplex DNA can be formed in a specific region of duplex DNA, and its biotechnology applications will greatly expand. In this study, we designed and synthesized novel C-nucleoside analogues based on the pyrimidine skeleton, 3MeAP-d(Y-Cl) and 3MeAP-d(Y-H), capable of recognizing a CG mismatch site that is not recognized by natural nucleosides. After incorporating them into the oligonucleotides, their triplex forming abilities were evaluated by gel-shift assay. Although it was only one sequence, the 3′-GZG-5′ sequence, the stability of the CG mismatch site recognition was greatly improved compared with previous nucleoside analogues.
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