放大器
化学
等离子体子
肉眼
核酸
聚合酶链反应
病毒学
清脆的
检出限
分子生物学
色谱法
生物
生物化学
基因
光电子学
物理
作者
Yongya Li,Howaida Mansour,Tony Wang,Sudarsana Poojari,Feng Li
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2019-09-03
卷期号:91 (18): 11510-11513
被引量:127
标识
DOI:10.1021/acs.analchem.9b03545
摘要
Herein, we described a novel plasmonic CRISPR Cas12a assay for the visual, colorimetric detection of grapevine viral infections. Our assay generates rapid and specific colorimetric signals for nucleic acid amplicons by combining the unique target-induced incriminate single-stranded DNase activity of Cas12a with plasmon coupling of DNA functionalized gold nanoparticles. The practical applicability of our plasmonic assay was successfully demonstrated through the detection of emerging red-blotch viral infections in grapevine samples collected from commercial vineyards.
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