Research on the determination of molecular weight distribution and the peptide content of Ganlong capsule

Objective To establish the molecular weight distribution of Ganlong capsule by HPSEC the content of the peptide determined by Lowry and Methods The superdex peptide 10 /300 GL(10 mm × 300 mm) column was used.The p H = 6.0 and phosphate buffer of 0.05 mol / L was used as the mobile phase,containing 0.1 mol / L Na Cl.The flow rate was set at 0.7 m L / min; The column temperature was 25 ℃; The detection wavelength was 214 nm.Results The content of the peptide ranged from 0.08 mg to 0.4 mg(r = 0.9996).The RSDs of measurement precision of molecular weight and content were 0.08% and 0%(n = 6),respectively.The RSDs of the repeatability were 1.3% and 1.1%(n = 6); The regression equation of standard material was log Mr = 5.1455-0.0871 tR,r = 0.9983,the relative molecular weight ranged from 2.68 × 102 Da ~ 5.73 × 103Da(r = 0.9983).Conclusion on The method is simple and rapid for determining the peptide content and the molecular weight distribution of Ganlong capsule.It can be used quality control method for Ganlong capsule.