Technology of multi-enzymatic hydrolysis to Corbicula fluminea protein

Taking the degree of hydrolysis(DH) and bitterness evaluation as indexes,the result was obtained by single factor test and orthogonal experiment.The results showed that papain,neutrase and trypsin was more effective to hydrolyze protein of Corbicula fluminea.The optimum conditions with three enzymes(papain,neutrase and trypsin) hydrolysis was that papain,neutrase and trypsin were added simultaneously,the ratio of alkaline papain/neutrase/trypsin was 1∶2∶1(W/W/W),enzyme amount 0.7%(W/W),solid-liquid ratio 1∶2(W/V),pH6.5,at 55℃for 6.0 h.The degree of hydrolysis with three enzymes was over 5.92%.The bitterness score was 4.The hydrolysate produced was luster-transparent,better flavour,less smell or bitterness.