明胶
丝胶
材料科学
丝绸
生物高聚物
组织工程
肿胀 的
化学工程
生物医学工程
复合材料
化学
聚合物
生物化学
医学
工程类
作者
Biman B. Mandal,Anjana S. Priya,Subhas C. Kundu
标识
DOI:10.1016/j.actbio.2009.03.026
摘要
In this study, we report for the first time the fabrication of novel 3-D sericin/gelatin scaffolds and 2-D films using non-mulberry Antheraea mylitta silk cocoon sericin protein. The matrices were fabricated, biophysically characterized and optimized for cell culture applications. Blended sericin/gelatin 3-D scaffolds were highly porous with an optimum pore size of 170 ± 20 μm. The scaffolds were robust with enhanced mechanical strength and showed high compressibility. Swelling studies showed high swellability along with complete degradation in the presence of phosphate-buffered saline. Cytocompatibility of the matrices was evaluated using feline fibroblasts showing normal spreading and proliferation as assessed by fluorescence microscopy. Cell cycle analysis showed cytocompatibility without any cell cycle arrests. Low immunogenicity of the matrices as observed through tumor necrosis factor α release reveal its potential as future biopolymeric graft material. The results of this novel study lay the foundation for the use of the silk cocoon protein sericin as a biocompatible biopolymer for tissue engineering applications.
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