The essential oil from Rosmarinus officinalis L. collected in Dushan, Guizhou Province was analyzed qualitatively and quantitatively by GC-MS, and 29 compounds were identified. The main compounds are α-Pinene, Camphene, α-Phellandrene, β-Phellandrene, 1,8-Cineole, Camphor, Borneol,Bornyl acetate,β-Caryophylene, α-Caryophylene.