乙酰胆碱
微透析
5-羟色胺能
化学
兴奋剂
血清素
河豚毒素
海马体
5-羟色胺受体
皮质(解剖学)
内分泌学
内科学
受体
神经科学
细胞外
生物
医学
生物化学
作者
Maria Grazia Giovannini,Ilaria Ceccarelli,Beatrice Molinari,Marco Cecchi,Joseph Goldfarb,Patrizio Blandina
出处
期刊:PubMed
日期:1998-06-01
卷期号:285 (3): 1219-25
被引量:16
摘要
The modulation of acetylcholine (ACh) release by 5-HT3 receptor activation was studied using in vivo microdialysis. Spontaneous and K+-stimulated ACh release were measured in frontoparietal cortex and hippocampus of freely moving rats. Two consecutive exposures to high K+ produced ACh release of similar magnitude. In the cortex, serotonin (5-HT) failed to alter spontaneous ACh release, but caused a concentration-dependent decrease of K+-evoked ACh release. Phenylbiguanide (PBG) and m-chlorophenylbiguanide, two selective 5-HT3 agonists, mimicked the 5-HT responses, but 8-hydroxy-2-(di-n-propylamino)tetralin, a selective 5-HT1A agonist, was without effect. However, PBG failed to modify K+-evoked ACh release from the hippocampus. Systemic and local administration of a highly selective 5-HT3 antagonist, tropisetron ((3-alpha-tropanyl)1H-indole-carboxylic acid ester) blocked the effect of both 5-HT and PBG. The inhibition of ACh release by PBG was sensitive to tetrodotoxin. These observations provide direct evidence that, in rat cortex, 5-HT modulates in-vivo release of ACh through activation of 5-HT3 receptors.
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