细菌
抗生素
微生物学
抗生素耐药性
显微镜
细菌细胞结构
原子力显微镜
纳米技术
显微镜
抗菌剂
生物
材料科学
光学
物理
遗传学
作者
María Inés Villalba,Eugenia Rossetti,Allan Bonvallat,Charlotte Yvanoff,Vjera Radonicic,Ronnie Willaert,Sandor Kasas
标识
DOI:10.1073/pnas.2221284120
摘要
Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is challenging. Recent studies demonstrated that monitoring bacterial nanomotion by using atomic force microscopy (AFM) upon exposure to antibiotics constitutes a rapid and highly efficient AST. Here, we present a nanomotion detection method based on optical microscopy for testing bacterial viability. This novel technique only requires a very basic microfluidic analysis chamber, and an optical microscope equipped with a camera or a mobile phone. No attachment of the microorganisms is needed, nor are specific bacterial stains or markers. This single-cell technique was successfully tested to obtain AST for motile, nonmotile, gram-positive, and gram-negative bacteria. The simplicity and efficiency of the method make it a game-changer in the field of rapid AST.
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