Bioactive microsphere-based coating for biomedical-textiles with encapsulated antimicrobial peptides (AMPs)

In this study a sonochemical method was used to prepare chitosan-based-micro/nanoparticle with incorporated antimicrobial- peptides as a novel antimicrobial coating for cotton-gauzes. Characterization in terms of size, morphology and stability was evaluated and microspheres were approximately 2 μm in-size and were further coated by the layer-by-layer deposition of alginate (Alg) and chitosan (CS). In addition, an antimicrobial-peptide Dermicidin-1-L was incorporated into the microspheres (CS/AMP microspheres) to give more effective antimicrobial activity against a wider range of microorganisms. Results showed a much higher antimicrobial activity of the CS/AMP-microsphere-coated-cotton in comparison with LbL-Alg/CS-microsphere-coating, without cytotoxicity.