生物柴油
生物转化
响应面法
生物柴油生产
生物量(生态学)
制浆造纸工业
化学
中心组合设计
纳米-
化学工程
材料科学
生物技术
色谱法
催化作用
有机化学
生物
工程类
发酵
农学
作者
S. Balraj,Gnanaprakash Dhakshinamoorthy,J. Iyyappan,B. Bharathiraja
标识
DOI:10.1016/j.biortech.2023.130012
摘要
In this study, zinc oxide (ZnO) nano particle immobilized recombinant whole cell biocatalyst (rWCB) was used for bioconversion of waste fish oil in to biodiesel in a lab scale packed bed reactor (PBR). Central composite design and hybrid artificial neural network (ANN) models were explored to optimize the production of biodiesel. Developed rWCB exhibited maximum lipase activity at 15 % (v/v) of glutaraldehyde concentration and 6 % (w/v) of ZnO nanoparticles at pH of 7. Maximum biodiesel yield reached about 91.54 ± 1.86 % after 43 h in PBR using hybrid ANN model predicted process conditions of 13.2 % (w/v) of nano immobilized rWCB concentration and 4.7:1 of methanol to oil ratio at 33 °C. Importantly, developed nano immobilized rWCB was adequately stable for commercialization. Thus, production of biodiesel from waste fish oil using ZnO nano immobilized rWCB could become potential candidate for commercialization.
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