From isolation of human ovarian follicles to the artificial ovary: tips and tricks

Cryopreservation and transplantation of ovarian tissue has proved to restore both endocrine and reproductive functions in cancer patients. However, women with a high risk of ovarian involvement cannot undergo ovarian tissue transplantation due to possible recurrence of the disease. Therefore, different teams worldwide are now working on the development of experimental strategies, like the transplantable artificial ovary (TAO), with the noble goal to offer to these patients hope for future conception. This article discusses some essential points for the creation of a TAO and reviews the latest progress made.