THE STUDY ON THE EXTRACTION OF FISH SCALE COLLAGEN WITH PEPSIN

In the experiments, the extraction of the fish scale collagen with pepsin under the acid condition, concerning with three factors: E/S, fish scales protein concentration and extraction time in a mixed uniform design or a orthogonal design was studied. Finally the best condition of extraction with pepsin was determined: 30 mg pepsin to 1 g fish scale, 3 % acetic acid 80 mL, shaking at the constant temperature (18 ℃) for 48 h.