Progress of monoclonal antibody secretion hybridoma cell rapid screening technology based on HTS-ELISA

Various immune analysis technologies based on the monoclonal antibody are the main development direction for modern food safety rapid detection technology.Therefore,preparation of monoclonal antibodies with high-activity has got more and more attention by researchers.Preparation of monoclonal antibody usually involves many techniques,including the preparation of the antigen,immune design,cell fusion,screening of active hybridoma and antibody purification.Among them,the screening of monoclonal antibody secreting hybridoma cells is a key of the prepared monoclonal antibody part.Therefore,in order to provide a reference for high-activity monoclonal antibody preparation and the development of rapid detection technology for food safety,this paper focused on low density fusion cell culture,special equipment and the principle and technology of determining hybridoma cell activity in the screening process according to drag the number of holes(Trailing) to related activities,and mainly introduced one kind of high-activity monoclonal antibody secretion hybridoma technique which was based on HTS-ELISA screening method.