Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification

values exceeding 0.999, and a coefficient of variation (CV) for quantitative repeatability of less than 2% across various concentrations. These results verify the system's precision, stability, and reproducibility. The developed system significantly enhances the throughput and accuracy of ddPCR detection, and the proposed algorithm further advances the practical application of deep learning in digital PCR image analysis.