无鞭毛体
U937电池
巨噬细胞
杜氏利什曼原虫
利什曼原虫
青蒿素
生物
一氧化氮合酶
一氧化氮
墨西哥利什曼原虫
川地163
分子生物学
药理学
微生物学
利什曼病
免疫学
细胞凋亡
内脏利什曼病
生物化学
体外
恶性疟原虫
寄生虫寄主
疟疾
万维网
计算机科学
内分泌学
作者
Suhair Dakhil Neamah,Hayder Z. Ali,Mohammad M. F. Al-Halbosiy
出处
期刊:PubMed
日期:2022-01-01
卷期号:68 (2): 331-338
摘要
Leishmaniosis is a parasitic infection spreads to humans by sand flies. Over 20 different species of Leishmania are responsible for the disease, which infect over 14 million people around the world. Chemotherapy is one of the most effective treatments for leishmaniosis, however it is restricted by the high cost and/or toxicity. In this study, the possible effect of artemisinin (ART) was detected on intracellular amastigotes of Iraqi strain of Leishmania donovani in ex vivo condition in U937 macrophage cell line. Gene expression of inducible nitric oxide synthase (iNOS) in U937 macrophage was investigated, before and after treatment with artemisinin. Kinetic result by real-time PCR demonstrated that the iNOS expression folding reached the maximum at concentration of 500 μM after 24 hours, at 750 μM after 48 hours and at 1000 μM after 72 hours, which was 56, 11, and 6, respectively. The copy number of iNOS gene expression was also significantly higher in treated infected U937 cells compared to both non-treated-infected cells and intact macrophages, under different concentration of ART along the three times of follow-up. Moreover, stained macrophages with fluorescent DAPI proved that the percentage of intracellular infective amastigotes was decreased to the minimum in treated U937 cells, in comparison to non-treated cells. The minimal amastigote-invasion percentage was recorded at 1000 μM, which was 26%, 27%, 21% compared to 61%, 87%, 75% in untreated cells after 24, 48, 72 hours respectively. These findings demonstrated ART positive efficacy against iNOS expression and this compound can be further studied as novel therapeutic rather than toxic available chemotherapies.
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