对苯二酚
安培法
结直肠癌
B细胞激活因子
化学
免疫学
医学
电化学
癌症
电极
癌症研究
内科学
抗体
生物化学
B细胞
物理化学
作者
Beatriz Arévalo,Marina Blázquez‐García,Aránzazu Valverde,Verónica Serafín,Ana Montero‐Calle,Guillermo Solís‐Fernández,Rodrigo Barderas,Paloma Yáñez‐Sedeño,Susana Campuzano,José M. Pingarrón
标识
DOI:10.1016/j.bioelechem.2022.108157
摘要
This work reports the first dual magnetic beads (MBs) assisted immunoplatform for the simultaneous determination of BAFF (B cell activation factor) and APRIL (a proliferation-induced ligand), two cytokines related to immunity and tumour invasion, growth and metastasis. The electrochemical immunoplatform involves sandwich-type immunoassays implemented on magnetic microparticles functionalized with neutravidin (NA-MBs) or carboxylic groups (HOOC-MBs), and amperometric detection (Eapp = - 0.20 V vs. Ag pseudo-reference electrode) at screen-printed dual carbon electrodes (SPdCE) using the H2O2/hydroquinone (HQ) system. The developed immunosensors provide improved sensitivity, with LOD values of 0.33 and 16.4 pg mL-1 for BAFF and APRIL, respectively, and much shorter assay time that those claimed for ELISA kits and allow their simultaneous determination. The dual immunosensor permits discrimination between healthy individuals and patients diagnosed with Systemic Lupus Erythematosus (SLE) or colorectal cancer (CRC) through the determination of both cytokines in 100-times diluted human sera with results in agreement with those provided by the individual ELISA methodologies.
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