Using18F-Pen-peptide to detect cisplatin-induced apoptosis in human non-small cell lung cancer cells

1268 Purpose: The aim of the present study was to investigate the relationship between the accumulation of 18F-Pen-peptide and the apoptotic cells that induced by cisplatin in non-small lung cancer cells.Methods: After pre-treatment with 60ug/ml cisplatin for 24h,A549 cells were analyzed directly by flowcytometry analysis. Caspase-3 activity was quantified by fluorometric immunosorbent enzyme assay (FIENA).Values were presented as percent (%) of control cellsuntreated. Invitro, accumulation and blocking tests were completed. Results:Apoptosiswas found in A549 cells after cisplatin treatment.In control group without cisplatin, 3.72%-5.37% of cells of A549 cells was exhibitedapoptosis. The population of apoptotic A549 cells that treatedby cisplatin increased in a dose-dependent manner. The addition of cisplatin to A549 cell cultures increased the apoptotic cells up to 32%, reaching a maximum at 60ug/ml after 24h of incubation,depended on the dose of the reagent. Cisplatin using 60ug/ml induces apoptosis in a time-dependent manner.The activity population of apoptotic cells increased and reached a maximum at 18h after the cisplatin treatment, and there was a significantly decrease at 24h point.Cisplatin induced apoptosis increasing of A549 cells in a dose-dependent manner that also resulted in a significant increase of caspase-3 activity. Caspase-3 activity ofuntreated A549cells was between 10 and 70 units.Cisplatin also inducedcaspase-3 activityof A549 cellsincreasing significantly in a dose-dependent manner in 24h.The activity population of apoptotic cells increased and reached a maximum 18h after cisplatin treatment, and there was a significantly decrease at 24h.The blocking study indicated that radioactivity in cells was reduced to background level after 60min administration of 18F-Pen-peptide.Conclusion:The apoptosis of A549 cells was inducedby cisplatinin a dose-dependent manner and the caspase-3 activitywas also increased in the same trend. The accumulation of 18F-Pen-peptide was related to the caspase-3activity levels in A549 cells. There was high specific interaction between the tracer18F-Pen-peptide and caspase-3 in non-small lung cancer cells.