化学
荧光
肉眼
水溶液中的金属离子
离子
部分
滴定法
检出限
金属
转身(生物化学)
质子核磁共振
分析化学(期刊)
光化学
无机化学
立体化学
色谱法
有机化学
物理
量子力学
生物化学
作者
Satyajit Mahata,Sandeep Kumar,Souradeep Dey,Biman B. Mandal,V. Manivannan
标识
DOI:10.1016/j.ica.2022.120876
摘要
The probe (L) having hydrazinecarbothioamide and 1,8-naphthalimide moieties was synthesized and evaluated for its metal ion sensing ability. It exhibits a selective and sensitive colorimetric as well as fluorescent recognition of Hg2+ and Ag+ ions in CH3OH - HEPES buffer solution (5 mM, 7:3, v/v, pH = 7.4) in presence of other metal ions. Probe L is weakly fluorescent upon excitation with 410 nm light, but after gradual addition of HgCl2 and AgNO3 enhancement in fluorescent intensity was observed. Detection limits of Hg2+ and Ag+ using L have been found to be 20 nM (Hg2+) and 40 nM (Ag+) over the pH range of 6 – 10 that is suitable for practical application under physiological pH conditions. The reversibility of interaction of L with Hg2+ ion was monitored using Na2EDTA by emission titration. The “OFF-ON’’ fluorescence switching can be observed with naked eye, in which fluorescent “OFF’’ is due to operation of PET process in free L. Whereas upon complexation with these two metal ions, PET is restricted and CHEF process becomes operational. Mass spectral analysis and Job’s plot yielded a binding ratio of 1:1 for both metal ions. NMR titration studies are consistent with binding of Hg2+ or Ag+ to NH group (attached with naphthalimide moiety) and sulfur atom of hydrazinecarbothioamide group. From cytotoxicity assay, 5 μM solution of L was considered in cellular imaging study and the potentiality of the probe L was established by using human breast cancer cell line (MDA-MB-231) and primary human dermal fibroblasts (HDF), through fluorescence cell imaging experiments for tracking both Hg2+ and Ag+ in living cells.
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