Exploring the Anti-Alzheimer’s disease mechanism of Xanthoceraside based on GEO database and Network Pharmacology

Objective: To explore the mechanism of Xanthoceraside in the treatment of Alzheimer's disease based on GEO data mining, Network Pharmacology and molecular docking technology.Methods: Obtaining the biological functional targets of Xanthoceraside through Pharmmaper and SEA data platforms.Screening: The differentially expressed genes of Alzheimer's disease on the GSE138260 datasets by R software limma package. Constructing the Wayne diagram of Xanthoceraside action targets and disease-related targets and screening the potential action targets of Xanthoceraside in the treatment of Alzheimer's disease. Using Cytoscape software and string database to construct the "key target pathway" network diagram and protein interaction (PPI) network diagram and enriching the GO and KEGG of the potential targets with the help of R language. Performing molecular docking between Xanthoceraside and the core target by PyMOL software. Results a total of 228 active targets of Xanthoceraside and 4836 differentially expressed genes of Alzheimer's disease Gene chips were obtained. 50 potential targets of Xanthoceraside in the treatment of Alzheimer's disease were obtained through the Wayne diagram. 10 core targets were screened out according to the degree value greater than the median. GO enrichment analysis revealed 57 biological processes, 17 cell components and 28 molecular functions and KEGG enrichment analysis revealed 33 related signaling pathways. Molecular docking testified that Xanthoceraside has stable binding ability with 9 core targets.Conclusion: Xanthoceraside has therapeutic effect on Alzheimer's disease mainly through multiple targets and pathways.