Determination of Lobetyolin in Radix Codonopsis Tubulosae of Guizhou

Objective Determination of lobetyolin in Radix Codonopsis Tubulosae of Guizhou was carried on to control the quality,introduction and cultivation,etc.Methods Hypersil ODS2(250 mm×4.6 mm,5 μm)was used with acetonitrile-water(20∶80) as the mobile phase and DAD detection was at 270 nm.Results Radix Codonopsis Tubulosae of 5 habitat in Guizhou were analyzed.The content of lobetyolin ranged from 1.265～4.093 mg·g-1.Conclusion The method appeared to be simple,quick and reproducible.It can be used to control the quality of Radix Codonopsis Tubulosae.