Effect of vascular endothelial growth factor from mesenchymal stem cells on anti-apoptosis of H9C2 cells through PI3-K/Akt signal pathway

AIML:To explore the effect of vascular endothelial growth factor(VEGF) from mesenchymal stem cells(MSCs) on anti-apoptosis of H9C2 cells.METHODS:In vitro,condition medium(CM) from MSCs was collected after MSCs were cultured for 2 days.After H9C2 cells were pretreated with VEGFR inhibitor SU5416(5 u,mol/L) or PI3-K/Akt inhibitor LY294002(10 μmol/L) for 1 h,apoptosis of the cells was observed under hypoxia with Annexin V and PI double staining by flow cytometry.The expressions of Akt and pAkt in H9c2 cells were detected by Western blotting and expressions of VEGF in MSCs were detected by RT-PCR.RESULTS:RT-PCR showed the expression of VEGF on MSCs,and Western blotting showed increased levels of pAkt protein in H9C2 cells after treatment with CM.CM decreased H9C2 cell apoptosis but the anti-apoptotic effect of the CM was blocked by VEGFR inhibitor SU5416 or PI3-K/Akt inhibitor LY294002(10 μmol/L),respectively.CONCLUSION:VEGF from MSCs plays an important role in the protection of H9C2 cells through PI3-K/Akt signal pathway.