Determination of chlorogenic acid and protocatechuic acid in Yinshanlian granules by HPLC

Objective To develop an HPLC method for the simultaneous determination of chlorogenic acid and protocatechuic acid in Yinshanlian(traditional Chinese medicines) granules.Methods Chromatography was performed on a Diamonsil C18 column(200 mm×4.6 mm,5 μm) with the mobile phase of acetonitrile-0.2% phosphoric acid solution(V∶V=10∶90).The detection wavelength was set at 285 nm and the column temperature was 30 ℃.Results The linear calibration curve were obtained in the concentration range of 4.80-43.2 mg·L-1 for chlorogenic acid and 4.08-36.7 mg·L-1 for protocatechuic acid.The mean recoveries of chlorogenic acid and protocatechuic acid were 101.8%(RSD=1.0%,n=9) and 98.5%(RSD=1.4%,n=9),respectively.Conclusions The method can be used to control the quality of Yinshanlian granules,which provides the advantage of quickness,simplicity and repeatability.