扩展器
低温保存
精液
精液冷冻保存
精子
特里斯
男科
精液扩展器
胶体金
化学
纳米颗粒
生物
精子活力
材料科学
纳米技术
细胞生物学
医学
生物化学
胚胎
有机化学
聚氨酯
作者
Aya A. Ismail,Adel Abdelkhalek,Wael A. Khalil,M. El-Harairy
出处
期刊:Journal of Animal and Poultry Production (Print)
日期:2020-02-01
卷期号:11 (2): 39-45
被引量:3
标识
DOI:10.21608/jappmu.2020.78854
摘要
There are various challenges during semen cryopreservation used for artificial insemination, oxidative stress is considered as the major factor. Usage of nano-particle materials is an efficient tool to overcome these challenges. In the present article, effects of adding green synthesized gold nanoparticles (GSGNPs, 5; 10ppm/mL) to Tris-extender on sperm characteristics in post-equilibrated and post-thawed semen. Apoptosis and sperm ultrastructure post-thawed were also examined as well as enzyme activity and antioxidant status in post-thawed extender of goat semen were evaluated. Supplementation of GSGNPs (10 ppm) increased (P<0.05) motility, livability, and membrane integrity post-equilibration, and also in addition to acrosome integrity post-thawing. Also, viable sperm increased (P<0.05), early apoptotic, apoptotic, and necrotic sperm decreased (P<0.05) by 10 ppm GSGPNs. In the extender of thawed semen, AST and ALT activities were not affected, total antioxidant capacity and catalase activity were higher (P<0.05), while malondialdehyde decreased (P<0.05) by 10 ppm GSGNPs. Frequency distribution of sperm cells with intact plasma or acrosomal membranes increased (P<0.05), while of those with swollen plasma membranes or typical acrosome reaction decreased (P<0.05). However, frequency distribution of sperm cells with slightly swollen or lost plasma membranes as well as atypical acrosome reaction and lost acrosomal membrane was not affected by GSGNPs. Transmission electron microscopy examination revealed no entry of GSGNPs with sperm cells.The present article may suggest considerable addition of GSGNPs at a level of 10 ppm/mL to Tris-extender of cryopreserved goat semen during cryopreservation.
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