毛螺菌科
代谢组
生物
胃肠病学
内科学
微生物群
代谢组学
肠道菌群
生理学
医学
16S核糖体RNA
细菌
生物化学
生物信息学
厚壁菌
遗传学
作者
Hongyu Liu,Xueqing Yin,Jiaying Li,Yan Cao,Yanjie Wang,Wenjing Mu,Zipeng Zhuo,Lu Chen,Zhongjie Zhang,Xutong Qu,Changsong Wang,Zhaodi Zhang
标识
DOI:10.1016/j.accpm.2022.101140
摘要
Delayed neurocognitive recovery (dNCR) is a common complication of the central nervous system in elderly patients. Currently, it is not clear whether the occurrence of dNCR is associated with the intestinal microbiota and its related metabolites. This study investigated the preoperative intestinal microflora and faecal metabolites of dNCR patients.Twenty-two elderly urological patients were divided into a dNCR group (D group) and a non-dNCR group (ND group) according to the postoperative Mini-Mental State Examination (MMSE) score on the first and third day after surgery. A postoperative MMSE score ≤ 2 points compared with the preoperative score was considered evidence of dNCR. We used a comprehensive method that combined 16S rRNA gene sequencing and untargeted metabolomics to study the preoperative intestinal microflora and faecal metabolites of the two groups, and conducted correlation analysis between them.Compared with the D group, the microbial community in the ND group was more abundant. At the family level, the ND group was significantly enriched in Lachnospiraceae, Peptostreptococcaceae and Muribaculaceae. At the genus level, the faecal microbiota of the ND group was differentially enriched in Agathobacter, Dorea, Fusicatenibacter, Coprococcus_2 and Romboutsia while that of the D group was differentially enriched in Anaerofilum. Untargeted metabolomics revealed significant differences in eight different metabolites between the two groups, including ribose, ethanol, leucine, maltose, pentadecanoic acid, malonic acid 1,3,4-dihydroxybenzoic acid and 3-hydroxypalmitic acid. In addition, differential metabolites were associated with the abundance of specific bacteria.The occurrence of dNCR may be associated with the intestinal flora and its related metabolite composition of patients before surgery.
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